Hmgb1 Knockout cell line(RAW 264.7)

Hmgb1 Knockout cell line(RAW 264.7) Order

Catalog#

YKO-M031

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

Hmgb1 Knockout cell line (RAW 264.7)

Morphology

monocyte/macrophage,adherent

Passage ratio

1:2-1:3

Species

Mouse

Gene Name

Hmgb1

Gene ID

15289

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10%FBS

Target region

Knockout region: Exon 3 (based on transcript Hmgb1-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:RAW 264.7				STR Info (Cell bank) Cell Line:RAW 264.7
Loci	Allele1	Allele2	Allele3	Allele4	Allele1	Allele2	Allele3
18-3	18.0				18.0
4-2	22.3				22.3
6-7	12.0				12.0
19-2
1-2	17.0
7-1	25.2				25.2
8-1	13.0				13.0
1-1					15.0	16.0
3-2	14.0				14.0
2-1	16.0				16.0
15-3	22.3				22.3
6-4	18.0				18.0
13-1	16.2				16.2
11-2	17.0				17.0
17-2	14.0	16.0			14.0	16.0
12-1	16.0				16.0
5-5	14.0				14.0
X-1	24.0				24.0
TH01
D5S818

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Hmgb1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the RAW 264.7 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Hmgb1 gene knockout Hmgb1 Knockout cell line (RAW 264.7) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

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